ABSTRACT
Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to the formation of a totipotent embryo. During the development of the pre-implantation embryo, precise reprogramming progress is a prerequisite for avoiding developmental defects or embryonic lethality, but the underlying molecular mechanisms remain elusive. For the past few years, unprecedented breakthroughs have been made in mapping the regulatory network of dynamic epigenomes during mammalian early embryo development, taking advantage of multiple advances and innovations in low-input genome-wide chromatin analysis technologies. The aim of this review is to highlight the most recent progress in understanding the mechanisms of epigenetic remodeling during early embryogenesis in mammals, including DNA methylation, histone modifications, chromatin accessibility and 3D chromatin organization.
Subject(s)
Animals , Female , Male , Mice , Chromatin Assembly and Disassembly , DNA Methylation , DNA Transposable Elements , Embryo, Mammalian , Embryonic Development/genetics , Epigenesis, Genetic , Epigenome , Fertilization/physiology , Gene Expression Regulation, Developmental , Histone Code , Histones/metabolism , Oocytes/metabolism , Spermatozoa/metabolismABSTRACT
Objective To explore the influence of acute myocardial infarction (AMI) patients with coronary lesions to the ST segment resolution (STR) and clinical prognosis after percutaneous coronary intervention (PCI).Methods 380 patients with AMI were detected twice each hour for the situation of STR,before and after coronary angiography and PCI.According to the STR,the patients were divided into STR (≥ 50％) group and no STR (＜ 50％) group.The relationship between STR and clinical prognosis of hospitalization or follow up were analyzed.Results The STR rate of patients with coronary multivessel disease or proximal lesions after PCI was lower than the rate of patients with coronary single vessel disease or distal lesions (P ＜ 0.05).The incidence rate of major cardiac events and LVEDd during hospitalization in patients with proximal coronary lesions was obviously higher than patients with distal lesions,while LVEF was lower than distal lesions(P ＜0.01).It had no significant differences in LVEF,LVEDd between coronary multivessl disease and single vessel disease during follow up (P ＜ 0.01).Conclusion It has significant relationship between coronary multivessel disease,proximal coronary,STR resolution in patients with AMI after PCI and the prognosis during hospitalization and follow up.
ABSTRACT
Objective To explore the relationship between ST-segment level,T-wave early inversion and patients' left ventricular function,prognosis specifically for ST-segment elevation acute myocardial infarction patients,which underwent emergency PCI.Methods 62 patients (M:40,F:22 ) with acute myocardial infarction were randomly divided into groups as follows:according to whether one hour ST segment's changes more than 50％ after PCI,they were divided into group A and B:group A (PCI after one hour ST segment ≥50％ ),group B (PCI ST segment after one hour ＜50％ ).According to whether 24-hour T wave inversion after PCI,they were divided into group C and D:group C (24-hour T-wave inversion),group D (24 hours without T wave inversion).After six months,the different LVEF betweeen group A and B,C and D was observed.Results Of group A and group C,after 6 months,the left ventricular function recovery was better than that of group B and group D ( P ＜ 0.05 ).Conclusion The analysis of ST segment and T wave are simple,practical and effective methods to predict myocardial reperfusion,especially for PCI therapy in patients with acute myocardial infarction.